After PQ exposure for 4, 8, 24, and 72 h, the bronchoalveolar lavage fluid was collected to determine levels of TNF- α and IL-1 β, and the arterial blood samples were collected for detection of PaO2 level.
The main pitfall of this analysis, however, lies in the fact that the data was not collected to measure levels of mobilization.
Serum samples were collected to assay levels of amylase enzyme, C-reactive protein, IL-6, catalase enzyme activity, malondialdehyde and pancreases were excised for histopathological examination and assay of pancreatic myeloperoxidase.
Additional samples were collected to evaluate levels of CRP and fasting lipids.
Following enrollment, a physical examination was performed and a blood sample was collected to determine levels of hematocrit and albumin and to assess platelet and leukocyte counts.
After desufflation, blood was collected to determine levels of plasma nitrite, NOS, inflammatory cytokines, and malondialdehyde, a marker of oxidative stress.
After chronic treatment of Lewis rats with the combination or individual components for 10 days, serum was collected to quantitate levels of corticosterone.
In particular, Barthel Index [ 23] and NIHSS National Institute of Health Stroke Scalee) [ 24] scores were collected to estimate the level of physical dependency and the level of neurological deficit, respectively.
Furthermore, differentiated 3T3-L1 adipocytes were exposed to 20 μM PAQG or vehicle for 48 h and then the cell sample were collected to detect the level of p-AMPK.
At the same time, the culture media were also collected to investigate the level of extracellular HMGB1.
