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Once empty, nests were collected to count parasites.
Every 24 h, 100 μL of cells was collected to count the number of cells in each well.
Blood was collected to count the number of leukocytes in the peripheral blood with a haematology analyzer (ERMA, Tokyo, Japan).
In 2009 and 2010, spikes of PA and PG trees at various stages were collected to count thrips (see Table 4 for the number of spikes).
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One month postpartum, maternal venous blood was collected to determine counts of CD4- and CD8-positive T-lymphocytes (CD4+ and CD8+).
Serum samples were collected to measure CD4 counts by flow cytometry.
The blood samples were collected to assess blood counts, liver enzymes, lipid profile and testosterone level.
Also, blood samples were collected to determine blood cell count and serum parameters (routineously performed in the Institute of Clinical Chemistry and Laboratory Medicine of the University Medical Center Mainz, Germany).
A physical examination was performed and peripheral blood collected to determine complete blood count (CBC), electrolytes, glucose, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyltransferase (GGT), bilirubin, and lutein.
Murine blood was also collected to ensure comparable peripheral blood counts between groups.
One day after treatment, bronchoalveolar lavage fluid was collected to determine differential cell counts, lactate dehydrogenase (LDH), and protein.
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