The collected cells were hypotonically swollen with 75 mM KCl for 15 min, and then fixed with −20°C Carnoy's solution (75% methanol/25% acetic acid) for 20 min. The fixative was changed once and the cells in Carnoy's solution were dropped onto glass slides and air-dried.
Panicles from each individual were collected ~5 days before flowering (swollen boot with top most flower of panicle approaching emergence), at flowering (50% of panicle emerged from boot), as well as 5, 10, 15, 20, 25, and 30 days after flowering.
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Colcemid-treated cells collected by mitotic shake-off were swollen in hypotonic buffer (0.03 M sodium citrate) at 37°C for 25 min and re-suspended in freshly prepared 3 1 mix of methanol:glacial acetic acid.
For observations prior to flowering, panicles were collected when the boot, or flag leaf sheath, was swollen yet before flowers had begun emerging.
Clinical measures including DAS28, the Health Assessment Questionnaire, tender joint count, swollen joint count and adverse events were also collected at baseline and every 4 weeks thereafter.
In 33% of the Tbx3 mutant embryos collected at E10.5 (n = 21), the pericardiac cavity was swollen and the heart incompletely looped (Fig. 5C,D).
Bursting axillary buds (DBO) were collected from the upper part of the stem and they swelled with emerging leaf primordia.
Data were collected on demographic variables, joint symptoms and tender (n = 68) and swollen (n = 66) joint counts.
The 31 poems of the Silesian issue of Čas (1903) had swelled to 88 by the last edition of the collected Slezské písně (1956; "Silesian Songs").
