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Note that chemical treatment alone for 10 min (S1) yielded significantly lower cell counts than other protocols, being the obtained cells also statistically less viable than any of the other cell collected suspensions.
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The band gap energy has been evaluated using the absorption data to characterize the likely structures of the particles in the collected suspension.
Absorption and fluorescence spectroscopies have been performed with the collected water suspensions.
After 2 h, we collected the suspensions containing root exudates and filtered them through 0.22-μm filter membranes to remove root detritus and microbial cells.
The collected cell suspensions were washed thoroughly with the phosphate buffered saline (PBS) and centrifuged twice at 3000 rpm for 5min to remove the culture media.
After sorting, the collected cell suspensions were either plated on solid LB agar containing the appropriate antibiotics or re-grown overnight for further rounds of analysis and cell sorting.
An equal volume of Luria-Bertani medium [34] containing 30% glycerol was added to the collected cell suspensions, which were then stored at −80°C as the mutant library of the new generation.
Detached cells were pooled with the previously collected cell suspensions and counted by staining with trypan blue.
The transfected cells in a 24-well plate were collected by suspension with PBS (500 µl/well).
The bones were flushed through with ice cold KRG without Ca2+ and the collected cell suspension was filtered through a 40 µm nylon cell strainer and pelleted by centrifugation at 200 g.
We then added 1 ml ethanol to the well and collected the suspension in a 2-ml centrifuge tube.
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