To extend to a lower level of similarity, the PSI-BLAST program [ 38] was used to collect successively sets of related proteins.
The IVGTT glycemia and insulinemia profiles of a diseased patient are collected and, successively, its metabolic parameters are obtained by fitting a compact delay model to those data.
Samples were successively collected at 0 4 min, 4 7 min, and 7 10 min after stimulation.
Supernatant medium was collected and centrifuged successively at 1,200 g and 10,000 g to pellet cells and large debris and finally at 100,000 g to collect apoptotic bodies.
Spectra shown here were recorded as soon as successively collected spectra (each recorded over 80 s) stabilized, indicating little further evaporation of liquid water.
Tissues from 51 adult patients harbouring glial tumours were successively collected during surgery at the Department of Neurological Surgery, University of Poitiers, France, with signed informed consent of all patients and the approval of the ethics committee of the Poitou-Charentes area.
After intravenous injection of 0.15 mL of 5% fluorescein-isothiocyanate (FITC-) labeled dextran (molecular weight 150,000), microscopic images of the muscle and the skin were successively collected, and the capillaries were quantified with Saisam software (Microvision, Evry, France).
After centrifugation, cells from the upper layer were successively collected, washed in PBS, centrifuged at 300 g for 10 min at room temperature, fixed in 4% paraformaldehyde/PBS solution for 15 min at room temperature, smeared on SuperFrost®Plus glass slides (Menzel Glaser, Braunschweig, Germany), air dried and finally stored at -20°C until further analysis.
Each image was collected in successive frames of 1 2 μm increments on a Z-stack plan at the appropriate wavelengths for secondary antibodies with a 40× plan apochromat objective and successively projected to obtain a double-stained 3D digital image by a computerized system (LCS lite, Leica Microsystems, Heidelberg, Germany).
The cells were then collected by centrifugation and successively washed with 100 mM EDTA, 30 mM EDTA and distilled water (with all procedures carried out under sterile conditions).
