In addition, 13 sediment cores were collected, sectioned into 5 10-mm slices and separated into pore water and solid phase.
Eight weeks postinfection, lungs were collected, sectioned, and stained by immunohistochemistry for various ER stress markers.
Collected Sections were homogenized in TRIzol (Invitrogene, Carlsbad, CA) by using an Ultra-Turrax homogenizer (Janke and Kunkel, Staufen, Germany).
8 days following CVB3 infection, heart and pancreas tissues of mice were collected, sectioned and subjected to HE staining.
Lungs were collected, sectioned, stained and scored as previously described.
Knee joints were collected, sectioned and stained 12 weeks post-surgery (n = 5 in each group).
After exsanguination, white adipose tissues (epididymal, mesenteric, subcutaneous inguinal and retroperitoneal), liver, total gut and muscle were collected, sectioned, weighed and stored in liquid nitrogen.
On 2 January 2013 we collected, sectioned, and stained interscapular brown adipose tissue, subcutaneous fat, and abdominal fat from a laboratory mouse (fig 1D-F).
Ovarian follicles at different developmental stages were counted in collected sections of an ovary, based on the well-accepted standards established by Pedersen and Peters.
