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Protein aggregates were pelleted at 15.500 g for 15 min. The supernatant was collected (S) and the pellet was washed, resuspended in SDS-containing lysis buffer and sonicated 4× at 50 Hz (P).
After incubation in this buffer, the supernatant was collected (S) and the insoluble proteins were pelleted.
The collected S exons were identified in the set of RefSeq scaffolds.
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In this way the designated sink collects s(s−1)/2 max-min positions.
One frame was collected every 2 s.
Scattering patterns were collected for 30 s per sample.
Data were collected every 10 s at 25 °C.
Phosphorous spectra will be collected at 10 s intervals.
VS-S collected, analyzed and interpreted the data.
All the parameters were collected at 2-s intervals.
Fluorescence data were collected every 5 s.
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