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During the spacewalk, the astronauts took pictures of the hole and collected residues that may help solve the orbital whodunit.
Cameron L. McNeil, the graduate student at the City University of New York who is organizing the seminar, has collected residues, including the one at the museum, from ceramics found in the tombs of the first rulers of Copán, a Maya city-state in Honduras founded in the fifth century.
Contact samples were taken from the inner surface of clean fermentation tanks before filling them with grape juice, and 1000 L of air inside the cellar was also filtered and the collected residues were plated as above.
The collected residues were screened using a gyratory screen (Black-Clawson) equipped with a 1.75-inch diameter round hole punched plate top deck to remove oversized particles and a 1/8-inch clear opening woven wire bottom screen (6 wires/inch mesh) to remove fines.
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The collected residue was dried at RT, then ground to fine powder in a mortar with pestle, and further dried in a temperature controlled vacuum oven at 70 °C overnight, to ensure complete removal of CHCl3.
The collected residue was used for DNA extraction.
We pumped 1000 L of vineyard air surrounding the grapes through a Millipore M Air Tester T (Millipore, USA) and plated the collected residue on RBCA as above.
Following the indication from NMR spectroscopy that there is differential mobility upon denaturation upon comparison of CP versus TM and EC regions in rhodopsin, we collected residue-specific information about the mobility of three residues within the predicted folding core in the EC domain, T108R1, V204R1, and I205R1 (colored red in Figure 5A and B1).
The precipitation is moved to the outside of the vessel through a small diameter pipe, in which any increase of water level in the vessel can cause the flow and together clearing the collected residue out of the container.
Their work was a mix of brute strength and finesse, alternately requiring moving debris and manipulating small detection kits, which involved breaking open jelly-bean-size ampules or mixing fluids and collecting residue with cotton swabs.
A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L). field near Ames, IA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com