Arriving home in the evening with a freshly collected baby, relaxation was replaced by playing, feeding, bathing and putting to bed.
The collected NMR relaxation data, together with the low number of NOE restrains for Glu221 and Ser222, additionally confirmed an increased mobility, and the disruption of helical conformation at those positions (Figures 3, 4A and 4B).
To further understand the motions associated with this acceleration, we collected N relaxation dispersion data for 14 0-, 15:0-, and 16:0-ACP.
The N T1 data were collected with relaxation delays of 10, 110, 190, 320, 400, 520, 630, and 750 ms. The N T2 data were collected with relaxation delays of 8, 32, 64, 96, 128, 192, and 248 ms. The N heteronuclear NOE data sets were acquired with the unenhanced and enhanced spectra interleaved and a 3 s proton excitation period for the NOE-enhanced spectrum.
Unfortunately we have collected the CPMG relaxation dispersion data at 500 MHz but the quality is very poor.
N Heteronuclear NOE (nuclear Overhauser effect) experiments were collected with a relaxation delay of 5 s with and without saturation of the amide protons that was achieved using 120° high-power pulses [ 16].
b 25Mg static variable temperature spin lattice relaxation data collected at 7.02 T plotted as function of temperature and Arrhenius fit (blue line).
We also collected 15N NMR backbone relaxation data for V22-SH3 wereh were very enlightening to pinpoint the dynamics of the local environment of a protein on the pico- to nano-second timescale.
N T2 data were collected with 10 different relaxation delays of 20, 40, 60, 80, 100, 120, 140 and 160 ms including repeats for 80 and 160 ms delays.
In the following generation 17, after one generation of relaxation, we collected 500 unsexed adult beetles from each of these experimental lines to produce offspring that were later used in the cross-infection experiment.
