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Serum was collected, protected from light and stored in −70°C until analysed.
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Ten-milliliter samples of heparinized peripheral blood were collected, immediately protected from light, and stored at 4°C for evaluation.
Furthermore, trough PK samples were taken before drug administration on days 8 and 22. Blood samples were collected and protected from light in EDTA-Vacutainer® tubes and plasma was separated by centrifugation and stored at −20°C within 2 h of collection.
Venous blood samples were collected after a 12-h overnight fasting, protected from light, centrifuged and stored at −80°C until analysis.
Venous blood samples were collected after a 12-h overnight fasting, protected from light, centrifuged and stored at −80°C until analysis was performed.
Urine samples were collected by cystocentesis and were also kept at 4°C and protected from light until analysis.
In each centre, blood for serum was collected at baseline, transferred to a local laboratory at 5-10 °C whilst protected from light, and following processing, stored in 0.5 ml straws at −196 °C in liquid nitrogen.
Fluorescein liposomes skin absorption was analyzed by collecting fluorescein samples directly from the receptor medium of Franz cells, protected from light, and measuring the concentration by fluorometry with a plate spectrophotometer (PerkinElmer Inc., Waltham, MA, USA; victor 3).
After collection, serum tubes were immediately put on crushed ice protected from light.
The solution was protected from light.
All experimental steps involving RES were protected from light.
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