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Cartilage stem/progenitor cells were counted and collected (passage 7) in logarithmic phase with conventional method.
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The resultant colonies were collected, passaged and the isolates were screened for proteolytic activity.
The first genetically related samples include early and high-passage samples (collected 30 42 passages after the early-passage samples) from three hESC lines, which we derived (the preimplantation-genetic-diagnosis [PGD]-derived hESC lines), and for which we have parental DNA from the blastocyst donors.
Mink EF were collected on passage 7, ES cells lines MES12 and MES29 on passages 21 and 25, respectively, iPS cell lines iNV7 and iNV11 on passages 10 and 11, respectively.
Samples collected at passage number 4 and 12 were included for all six SV40-Neo transfected and G418 selected clones.
■ Data to be collected: ○ Passage of cells, particularly pMEFs, injected in each mouse.
■ Sample delivered for further analysis: ○ Preserved primary tumors for further analysis in Protocol 4. ■ Data to be collected: ○ Passage of cells, particularly pMEFs, injected in each mouse.
HaCaT cells were collected at passage 43 for RNA isolation.
BMSCs in each group were collected at passage three.
Neurospheres formed after 1 week were collected for passage or further analyses.
This assay was performed after BMSCs were collected at passage three.
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