We collected data on seed rain and seedling recruitment from September 2015 until November 2016.
Seed weight was determined from three seed samples of 10 mg (around 400 seeds) collected on three individual plants.
Briefly, siRNA transfections were performed at 100 nM on days two and four post-seeding and cells were collected on day six post-seeding, forty-eight hours after induction of differentiation (see Materials & Methods for further details).
Numbers of weed seeds collected on the soil surface seems to be of greater importance than specific plant composition.
Total proteins were extracted as described by Gallardo et al., [ 63], from 20 mg of mature seeds collected on four individual plants.
The cells were collected on the fifth day, seeded into 24-well plates at a concentration of 10 cells/ml and incubated overnight with different concentrations of fluorescent NPs (0, 10, 20, 50, 150, 200, 300 and 500 μg/ml).
This is close to the Brazilian Association of Seeds and Plants' (2009) estimate that royalties are not collected on approximately 28%% of wheat seeds.
From September 2014, data were prospectively collected on all patients undergoing iodine seed tumour localization.
These seeds were collected by D.R. Sowell from a common garden experiment in Oxford, England, but the maternal plant was derived from seed originally collected on the Apple Orchard Falls Trail in Bedford County, Virginia, USA.
Part of the intra-taxon variation depended on the collection date (Tables 3 and 4) or starting point in the cycles (Table 4), but there were also substantial differences between seed batches collected on the same day and subjected to the same treatment (Figs 1 and 3).
