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Demographics and baseline clinical characteristics were collected on enrollment.
A convenience selection of serum samples collected on enrollment was used in the various assays of the present study, including a total of 189 serum samples from primigravidae, 21 from secundigravidae and 72 from multigravidae.
Demographics and baseline clinical information were collected on enrollment.
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To distinguish between recrudescence and reinfection, malaria parasites collected on day of enrollment (day 0) and day of recurrence were genotyped by RFLP analysis of a 700 bp PCR product of Pfmsp2 [26] and DNA sequencing a 400 bp PCR product from Pfmsp1 block 2 [27].
Demographic, anthropometric, medical, and diabetes history data were collected on participants at enrollment.
Parasite DNA was extracted from 200 µL of whole blood collected on the day of enrollment from a three ml Vacutainer (Becton Dickson) with EDTA using QIAamp® DNA blood mini kit (Qiagen, Valencia, Ca) according to manufacturer's instructions.
Baseline demographic data are collected on the day of enrollment by study personnel through personal interview.
Baseline medical history is collected on the day of enrollment by study personnel through personal and medical record review.
Information on exposure to SHS and maternal smoking during pregnancy was collected on 3,318 participants at enrollment into the Southern California Children's Health Study.
APACHE II, SOFA, SIRS and both the JAAM and ISTH DIC scores were assessed, and the platelet counts and coagulation variables necessary to diagnose DIC were collected on the day of enrollment (day 1).
Data for hemodynamic parameters, Sequential Organ Failure Assessment (SOFA) score, and blood sample were collected on first three day after enrollment The primary outcomes were leukocyte expression of CD11b, HLA-DR, chemotaxis function and blood endotoxin level on day 3 after enrollment.
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