Exact(5)
Cell lysates were then collected, normalized by total protein content and analyzed under reducing conditions with 5× SDS-gel loading buffer (50 mM Tris-HCl pH 6.8; 100 mM dithiothreitol; 2% (w/v) electrophoresis grade SDS; 0.1% bromophenol blue; 10% (v/v) glycerol on a 10% SDS-PAGE.
Data from 46 single microarrays (19th DLR PFC: 8x μg, 6x H/W, 8x 1 g, and 6x 1.8 g; TEXUS-49: 7x μg, 6x H/W, and 5x BL) were collected, normalized, and further analyzed.
To minimize the impact of clonal expansion on the VH gene usage profile, we collected normalized data, in which we scored each distinct IgH CDR3 nucleotide sequence expressing a given VH gene as one, no matter how many times this sequence was detected.
To minimize the impact of self-replenishment on the N-addition distribution profile, and hence to weight the repertoire for de novo generated IgH sequences for B-1a cells, we collected normalized data that counts each distinct IgH sequence containing indicated N nucleotide insertions as a single sequence, regardless how many times this sequence was detected.
Whenever available, we also collected normalized gene expression levels for a homozygously deleted gene of interest to see whether the gene is underexpressed compared with the rest of the samples in the same cancer study.
Similar(55)
Figure 1 shows the real-time user's emotion recognition process by collecting, normalizing and analyzing photoplethysmography (PPG), galvanic skin response (GSR) and skin temperature (SKT) data.
By collecting, normalizing, enriching, and connecting data that was previously siloed, these applications can recognize unseen patterns, foresee changes in demand, iron out manufacturing bottlenecks before they arise, and even replenish raw materials based on past purchasing patterns.
The collected and normalized quantified values do not distribute significantly different from the normal distribution (as tested by the D'Agostino-Pearson omnibus normality test, P = 0.1359, n = 175) and we could therefore use the t-test, with Welch's correction for unequal variances, for significance testing.
The expression data of 1,109 available grapevine kinase genes were collected and normalized.
The primers used were as Table 1.The cycle threshold (Ct) data were collected and normalized to β-actin.
The ability to recognize patterns and trends from data sources collected and normalized in the cloud will bring about innovations that will be substantial.
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