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The collected medium was concentrated with the PEG-it virus precipitation solution (SBI, catalog number LV810A-1), and pellets containing viral particles were resuspended with PBS.
The collected medium was diluted to 5 ml with PBS and further diluted by 15-fold with ethanol.
Albumin concentration in the collected medium samples was analyzed using a competitive enzyme-linked immunosorbent assay [ELISA].
This suspension was clarified by low speed centrifugation and the collected medium was pooled and stored at −80°C as virus stock.
At each passage level, the cells were collected on day 5-x post-infection, when CPE was apparent and freeze-thawed as described above, and the one-tenth of the collected medium was used to inoculate the next passage.
Briefly, at 0 min 10 µl aliquots of the inhibited AChE were transferred to cuvettes containing phosphate buffer (0.1 M, pH 7.4), 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) and 750 µl of the collected medium, which contains the transported oxime.
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Falling solid particle receivers (SPR) utilize small particles as a heat collecting medium within a cavity receiver structure.
Finally, there are the investment institutions, which collect medium- and long-term funds by issuing bonds and supply medium- and long-term credit for industry, public works, and agriculture.
The column effluent samples were harvested by collecting medium from the column outlet, by opening the quick release below the column.
Production of 3H2O was measured by collecting medium samples after 2 h and counting of the radioactivity as described (Giovannetti et al, 2008).
Production of H2O was measured by collecting medium samples after 2 h and counting of the radioactivity as described (Rots et al, 1999).
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