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He collected lichens.
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They collected lichen samples from trees, grave stones and benches in their local area and extracted DNA to identify the specific types of algae and fungi.
Freshly collected lichen tissue was air dried for ≥48 h prior to placing an intact 4 mg portion into 100 µL of dialyzed, HY BH.
We found that lichens collected at both sites were capable of degrading PrP and we compared the remaining PrP immunoreactivity from lichen-treated samples with non-treated samples (data not presented).
Interestingly, We also noticed the same correlation between heavy metals accumulation in soil and the concentration of some selected metals found by Cardarelli et al. in lichens collected in Rome in the same periods [23].
The data in Figure 9A are from lichens collected at the Eagle River site.
To study the distribution of the trace-elements between the lichens and the lichen throughfall water inside a virtual column, the throughfall water was collected under the lichen transplants during 6 and 12 months.
Over 2 weeks in 2007 and 2008, those volunteer climbers helped collect 394 lichen specimens.
For image analysis, five biological soil crust samples with different ratios of substrate, mosses and lichens were collected on an inland dune system in Lieberose, dominated by the moss Polytrichum piliferum, and the lichens Cladonia fimbriata and C. coccifera.
Fresh substrata samples of mosses and lichens were collected from walls in St. Maur des Fossés, Paris, France, a type locality for several tardigrade species.
Identical lichen species collected at different times or locations performed identically with respect to PrP degradation, further supporting the concept of species specificity.
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