The samples were collected, labeled, sealed, and stored following APHA (1995) standards in all cases.
We name the database that has the collected labeled video data and its protocols as the ANU Stress database (ANUStressDB).
Non-attached cells were collected, labelled with α-CD8-APC antibody and analysed by FACS.
The beads were then pelleted by centrifugation at 2000 g for one minute and the supernatant collected, labeled as "flow-through" and saved for future analysis.
A total of 1,167 butterfly individuals of 119 species were collected, labelled and later identified using D'Abrera [19], [20], Hecq [21] and Larsen [22].
Additionally, a small sample of M3 seed derived from each of the 27,000 unique M2 plants was collected, labeled, and frozen for long-term storage.
After incubation, the suspension was centrifuged at 4,000 g for 15 min and the supernatant collected, labeled as 4 M KOH extract and stored at 4°C.
Each screening day, on a random 10% subsample, a second blood sample was collected, labeled, and analyzed in a blind fashion to estimate the measurement error.
Twenty-four hours later, the young third leaves of randomly-selected plants from both the ABA-treated and control groups were collected, labeled as a1d (1 d after ABA-treatment) and c1d (1 d, control), respectively.
A separate aliquot of pleural fluid (in ideal circumstances, 10 mL) was collected, labeled according to the central coordinator's de-identification and specimen-tracking database, and stored at −20°C.
