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After 4 hours of collection, the needles are removed, and the total number of cells collected is determined by DAPI staining.
For PTB research, the biological sample collected is determined by the research question of interest; therefore a wide variety of fluids or tissues such as cervical mucus, blood, urine, saliva, vaginal discharge, myometrium, and uterine tissues are appropriate depending on the investigation.
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The approximate cell numbers collected were determined using a hemacytometer, and samples were then normalized to cell number (cell numbers never varied by more than 5%).
The amount of material collected was determined gravimetrically.
The FOV and frame rate for the images collected are determined by the amplitude and frequency of these control signals.
Additionally, the manner in which participant data was collected was determined to be ethically acceptable by the Institutional Review Board Services of Aurora, Ontario in Canada.
The concentration of the relevant ions in the supernatant collected was determined using ICP-OES (iCAP 6000 Series Thermo Scientific, Waltham, MA).
The iodide concentration of each aliquot collected was determined using an iodide-selective electrode (Thermo Scientific, Waltham, MA) and converted to iodide efflux rate (nanomol/min) as described previously.
Participants were recruited for individual interviews or focus groups until saturation was reached (data collected was determined to be rich and sufficient, and no new properties of the categories or theoretical insights were being gained) [ 19].
The proportion of Creb1−/− mice which were present in each litter at the stages collected was also determined.
Data collection is performed every 14 days from May to November and collected mosquitoes are determined to species level.
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