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In vacuum pervaporation, the global mass flux is classically estimated by weighing the mass of permeate collected in cold traps.
Briefly, 7 9 brain tissues (n = 4 litters) were harvested from postnatal (P3 P6) Sprague-Dawley rat pups (Charles River, Wilmington, MA) and collected in cold PBS.
The new mass flux method measurement was validated for single component permeation (ethanol and water) by comparing mass of permeate collected in cold traps with the level decrease of feed liquid measured with a pressure sensor.
The dissected tissue was collected in cold Neurobasal™ medium and rinsed twice with cold PBS.
Free-floating coronal sections (40 µM) were cut using a vibratome (Leica VT 1000S) and collected in cold PBS.
Pregnant C57Bl/6 (Harlan Interfauna Iberica, SA) females were sacrificed by CO2 inhalation, and embryos from E9.5, E11.5, E14.5, E15.5 and E18.5 stages were collected in cold PBS.
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At the maximum degradation rate, the products were collected in a cold trap and analyzed by a coupled gas chromatograph/mass spectrometer.
The individuals were collected in a cold zone associated with weak diffuse flow (< 10°C).
1 2 cm leaves were collected in ice cold imbibition buffer similar to described in [ 9] in a 50 ml falcon tube.
We asked whether repeated exposure to the tar fraction of CS, collected in a cold trap (TAR), promotes plaque development in an avian model of arteriosclerosis.
Under a dissection microscope, the vertebral column was opened and DRGs were dissected and collected in ice cold HBSS (Hank's Balanced Salt Solution: Invitrogen 14170-088).
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