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Similar observations were obtained from WB samples collected from three other donors.
Due to the lack of preserved frozen nodes from naturally scrapie-infected sheep which were used in the epitope mapping study, paired hemal and retropharyngeal lymph nodes collected from three other naturally scrapie-infected sheep (ARQ/ARQ Prnp genotype) were used for WB assays.
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The mating system was investigated using manual pollination tests on six clones, open-pollinated seeds were collected from four other clones to study gene flow and paternity analysis was done on progenies using microsatellite genetic markers.
Samples were collected from two other non-related groups to validate independently some of the results obtained.
Data were collected from two other 12 g solar-powered PTTs deployed on additional species to assess whether roosting density and roosting height influenced solar-powered PTT performance.
Secondly, we used data collected from four other hospitals in the same study period.
Further risk factor information was collected from two other mailed questionnaires and blood and tissue specimens were obtained.
Additionally, a duplicate grab sample was collected from six other facilities to allow for variations associated with the sampling procedure.
In addition to D. melanogaster and C. elegans, the modENCODE data also includes information collected from seven other drosophila and four other caenorhabditis species.
In addition to two experimentally scrapie-infected sheep (4489 and 4494) used in the epitope mapping study, paired hemal and retropharyngeal lymph nodes collected from four other blood transfusion recipients (VRQ/VRQ Prnp genotype) were also included in WB assays.
Data were collected from three independent repeats.
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