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In total, 193 serum samples were collected for the detection of antibodies against ORT using a commercial testing kit.
FLS were trypsinized and collected for the detection of apoptosis by using Annexin V-FITC Apoptosis Detection Kit (eBioscience).
A total of 390 samples from cows (195 faecal and 195 milk) and 22 stool samples from humans were collected for the detection of Salmonella.
To follow the success of the programme in an individual herd, 15 20 blood samples were collected for the detection of antibodies to M. hyopneumoniae.
Upon admission, rectal swab with visible stool content was collected for the detection of multidrug-resistant organisms including VRE in accordance to the hospital infection control policy.
Goose primary epithelial related tissues derived from small intestine, kidney, liver, lung, proventriculus, and cecum were collected for the detection of goIFNLR1 expression by qRT-PCR.
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They were subjected to three successive transfers of cultures on PDA plates containing 200 μg/mL hygromycin B; then, transferred into PDA plates for three consecutive cultures; and were later transferred into PDA plates containing 200 μg/mL hygromycin B. After the transferring cultures, the conidia were collected for the PCR detection of target fragment and Hph r gene.
Existing chiller data collected for the design of automated fault detection and diagnosis methodology is used.
HUVECs were incubated with PV and acetylcholine (ACh) for 1 h, and then the culture medium was collected for NO detection with the Griess reagent.
After 24 hours, whole-cell lysates prepared with passive lysis buffer were collected for reporter detection by the Dual Luciferase Reporter System (Promega) according to the manufacturer's protocol.
The application of different non-invasively collected matrices for the detection and quantification of environmental exposure to metals has abundantly been described in literature.
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