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Following the 8-week intervention, rats were euthanised 24 h following their last bout of exercise and tissues were collected for subsequent analysis.
The gel samples were then separated by centrifugation at different time intervals, and the supernatant liquid was collected for subsequent analysis using an UV-vis spectrometer (752, Sunny Hengping, Shanghai, China).
Fractions were collected for subsequent analysis using a Gilson 201 fraction collector.
Pups were euthanized at 72 h, and the small intestine and colon were collected for subsequent analysis.
The medium was replaced every other day and the resulting EBs were differentiated for different time points and collected for subsequent analysis.
Preoperative and postoperative values were collected for subsequent analysis.
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Hypy has the additional advantage that the non-BC fraction removed from a sample can be quantitatively collected for subsequent further analysis.
Finally, after these three steps, tags with matching information were collected for subsequent biological analysis.
Excised brains were then collected for subsequent immunohistochemistry analysis.
The corresponding supernatants were collected for subsequent protein analysis and stored at −80°C until use.
Tubes were centrifuged and the plasma collected for subsequent hormone analysis.
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