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On the day of fat injection, 18 days after the fat injection, and at the end of the study period, each mouse was weighed, and a tail vein blood sample was collected for determining the red blood cell, leukocyte, and platelet counts, the plasma hemoglobin, VEGF, and EPO concentrations.
Sections of fresh liver samples were collected for determining the lipid content.
The supernatant was collected for determining the protein concentration with a Plusone 2-D Quant Kit (Amersham Pharmacia Biotech, Uppsala, Sweden).
Then, the solution was centrifuged at 16,000 rpm for 15 min, and the supernatant was collected for determining the amount of free Dox.
Preceding the insulin clamp, overnight fasting blood samples were collected for determining the concentrations of fructosamine, glucagon, cortisol, NEFA, total cholesterol, LDL and HDL cholesterol and background glucose enrichment.
In addition, the anthers after their pollen dispersal were collected for determining the level of anther cracking and the amount of pollen left per anther by the above-mentioned method.
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After centrifugation at 3,000 g for 10 min, the supernatants were collected for determining amounts of pentoses and hexoses released from enzymatic hydrolysis.
The lysates were centrifuged at 13000 r.p.m. for 10 min at 4 °C and the supernatants were collected for determining protein concentration using a BCA protein assay reagent (Thermo Scientific, Waltham, MA, USA).
Access affects analysis by determining the hypotheses that can be tested and whether sufficient information can be collected for determining rates and predictors of exposure.
The cell pellet was resuspended in 5% metaphosphoric acid (Sigma-Aldrich) and supernatant was collected for determining total glutathione and oxidized glutathione (GSSG) concentrations using a commercial kit according to the manufacturer's protocol (glutathione assay kit; Trevigen Inc., Gaithersburg, MD).
Sewage samples were collected for determining DO.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com