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HTR8 cells and 1 ml-conditioned media were collected for confirmation of overexpression of miR-661 by RT qPCR.
In addition to RDTs, a reference slide (thick and thin blood smears) were collected for confirmation of parasitemia.
From animals with positive TST results, blood samples were collected for confirmation with an interferon-gamma release assay (BOVIGAM; Prionics, La Vista, NE, USA).
If the HIV test report was positive in the rapid HIV test, a second venous sample was immediately collected for confirmation of HIV infection and women were called back for the test report after 1 week.
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In such cases, a thick blood smear was also collected for subsequent confirmation of the diagnosis.
Tissues from ES cell derived pups identified by eye pigmentation at birth were collected for genotype confirmation and chimera-transmitters were immediately mated with C57BL/6N females to establish mutant strains on B6 background.
During follow-up, when a laboratory technician was not available to read a thick blood smear for malaria parasites, the Core™ Malaria Pf test (CORE Diagnostics, Birmingham, UK) was used to guide treatment and, a thick blood smear was collected for subsequent confirmation of the diagnosis.
On rare occasions during follow-up, when a laboratory technician was not available to read a thick blood smear for malaria parasites, the Core™ Malaria Pf test (CORE Diagnostics, Birmingham, UK) was used to guide treatment and a thick blood smear collected for subsequent confirmation of the diagnosis.
Cloacal swabs of affected flocks were then collected for laboratory confirmation.
Cervical tissue was also collected for histological confirmation of oestrous cycle.
Patients were routinely interviewed about a wide variety of potential exposures, and stool samples were collected for laboratory confirmation.
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