All animals were collected for analysis of total silver concentrations.
Water samples were collected for analysis of selected physicochemical parameters following water quality assessment protocols.
Every 4 wk, jugular blood was collected for analysis of metabolites and metabolic hormones.
Soils from 0 5 cm depth were also collected for analysis of organic compounds.
At each irrigation sewage effluent and bore well water were collected for analysis of BOD, COD and physico-chemical parameters.
Daily blood samples were collected for analysis of FSH, LH, estradiol, progesterone, and inhibin by radioimmunoassay (RIA).
During wk 20 of the feeding period, blood samples were collected for analysis of plasma fatty acid profiles.
Two liters of water samples were collected for analysis of nitrate nitrogen (NO3 N), Orto phosphate (PO 4 3 ), and total Nitrogen (TN), and chloride concentration in laboratory.
In order to do that, the sea water samples at 6 transects (18 stations) were collected for analysis of suspended sediment concentration (SSC) during pre-monsoon and post-monsoon periods.
During the last 2 d of each period, milk samples were collected for analysis of protein and fat content and feed samples of fresh feed and orts were collected for particle size analysis.
Plasma was collected for analysis of leptin and insulin per the instruction of the suppliers (Linco Inc. St. Charles, Missouri).
