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The reuterin-containing solution was collected, filter sterilized, and stored at 4 °C until further analysis.
Briefly, PyMT conditioned medium was collected, filter sterilised using a 0.45 μm filter, and stored at −80°C until use.
Viral supernatants were collected, filter sterilised (0.45 μ) and titrated in the range of approximately 10 PFU per ml.
LDMIII cultures were incubated anaerobically at 37 °C for 24 h, then supernatants were collected, filter sterilized using polyvinylidene fluoride membrane filters (0.22 μm pore size; Millipore, Bedford, MA) and stored at −20 °C before further processing.
From the 1,578 collected filter paper samples in each district, a randomization list was generated and a subsample of 372 (186 from the SMC+ group, mean age 6.2 ± 1.4 [93 positives + 93 negatives by microscopy] and 186 from SMC− group, mean age 6.3 ± 1.5 [93 positives + 93 negatives by microscopy]) was selected for serological analysis.
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At 48 h after transfection, viral supernatants were collected, filtered, and stored at −80 °C.
The water used for washing one barrel is collected, filtered and reused.
The suspensions were centrifuged (1400 g, 10 min) and the supernatant collected, filtered (0.2 µm pores) and stored at −80 °C.
From our location to our social connections and electronic conversations, the metadata of our nation is being collected, filtered and analysed.
Forty-eight hours post transfection, the media containing the virus was collected, filtered, and centrifuged at 70,952 × g for 2 h at 4 °C.
The report says: "Our privacy rights as individuals are engaged whenever these agencies embark upon such intelligence activity, including when the public's data is accessed, collected, filtered and eventually examined by an intelligence analyst.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com