Exact(12)
Collected extracts were immediately subjected to protein extraction.
Depending on the packing density of the extraction cells, the volume of the collected extracts was between 25 and 31 mL.
This acetone/hexane extraction step was repeated three times, and the collected extracts were combined.
The collected extracts were evaporated to an amount of approximately 20 μL.
Few drops of diazomethane in ethyl ether solution was added to the collected extracts, according to the previously described procedure.
The extent recovery of glucose from MIPs was analyzed by spectrophotometery of collected extracts at the wavelength of 490 nm using the procedure reported elsewhere [28].
Similar(48)
The extraction procedure was repeated twice and the collected extract was evaporated to dryness under vacuum at 40 °C by using rotary evaporator.
The collected extract was then concentrated using a rotary vacuum evaporator (R-215, Buchi, Switzerland).
These samples were extracted, as described earlier, except that the internal standard was added to the collected extract.
The extractability was measured as the mass ratio of the recovered protein in the collected extract solution compared with that in the 20 g of starting material.
A total of 51 blood DNA samples from 26 patients with migraine and 25 matched healthy controls were collected, extracted and treated with bisulfate.
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