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The extraction procedure was repeated twice and the collected extract was evaporated to dryness under vacuum at 40 °C by using rotary evaporator.
The collected extract was then concentrated using a rotary vacuum evaporator (R-215, Buchi, Switzerland).
These samples were extracted, as described earlier, except that the internal standard was added to the collected extract.
The extractability was measured as the mass ratio of the recovered protein in the collected extract solution compared with that in the 20 g of starting material.
The collected extract was then concentrated using a rotary vacuum evaporator, and transfer the extract into a cleaned and rinsed vial by rinsing with DD hexane for three times and making the volume 7 mL.
The collected extract was then concentrated to a smaller volume of 5 ml under reduced pressure using a rotary vacuum evaporator (Rotavapor-R 215, Buchi, Switzerland) based on the method described in the Deutsche Forschungsgemeinschaft (Thier and Zeumer 1987).
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Once data across that broad range is accessed and collected, extracting useful data from the noise requires sophisticated presentation, often relying on the best discrimination tool we know of: human attention.
A total of 51 blood DNA samples from 26 patients with migraine and 25 matched healthy controls were collected, extracted and treated with bisulfate.
TKO, AMV, BR, YA, CTRR and RV collected, extracted and quantified DNA.
Biliverdin was collected, extracted and purified as described above for the 2L bioreactor batch cultures.
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