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Ears were collected, embedded in paraffin and the fixed sections were immunostained.
For all other histologies, tissues were collected, embedded with OCT matrix, and immediately frozen in isopentane cooled in liquid nitrogen.
Ovaries from 4-month-old Tg females (n=3) were collected, embedded into Neg-50, and cut by a cryostat.
For SA- βgal staining in tissues, 21 days following tumor inoculation, mice were euthanized and tumors collected, embedded in OCT, flash frozen in liquid nitrogen and stored at −80 °C.
Four and eight weeks postsurgery, the animals were sacrificed, and the implanted hydrogel along with surrounding skin tissues were collected, embedded in polyfreeze, and then flash frozen in liquid nitrogen.
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Two FAA-fixed samples of each collected plant were embedded in paraffin (Johansen 1940) for transversal and longitudinal sections, respectively, and cut at 12.5 µm using a Microm rotary microtome (Thermo Fisher Scientific, Inc., USA).
After 7 weeks, the mice were euthanized with CO2 inhalation, organs were collected and embedded in Tissue-Tek CRYO-OCT and snap-frozen or fixed in 10% formalin and paraffin embedded.
Once the sample panel was back on Earth, the problem quickly became finding any collected particles embedded in the aerogel.
Upon euthanasia, tumours were collected and embedded in TissueTek (Sakura Finetek, USA Inc., Torrance, CA, USA) and frozen for autoradiography.
After 2 h, lungs were collected and embedded with the Cryo-Gel medium (Instrumedics).
After BrdU addition, the cells were collected and embedded in paraffin for BrdU and other immuno-labeling processes.
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