The hearts were collected, cut into 5-µm-thick sections, stained immunohistochemically for CD45, CD68, CD3, CD8, CD4, and αSMA, and analyzed by confocal microscopy.
Eight hours later, mice were killed, colon collected, cut in sections and FITC-conjugated Smad7 antisense oligonucleotide distribution assessed by immunofluorescence.
For this purpose, liver, kidney, spleen, heart, lung, and brain tissue samples were collected, cut into sections of approximately 0.5 cm sizes, and fixed in 10% formalin for at least 48 hours.
The vertebral bodies adjacent to the uppermost disc analyzed for the presence of CK8-positive cells were collected, cut mid-sagitally, and incubated with freshly prepared 1% toluidine blue solution (Sigma-Aldrich).
For each fruit, three tissues (skin, underskin flesh and pulp) were separately collected, cut in small pieces, immediately frozen in liquid nitrogen, ground into a fine powder and stored at -80°C until final processing.
The inner parts of galls were collected, cut into small cubes, and placed in fixation solution containing 2.5% glutaraldehyde and 4% paraformaldehyde in 0.1 M sodium phosphate buffer (pH 7.0) in the field.
Twenty-four hours after MCAO, cortical tissues from two rats of each treatment group (model, sham, 15, 30 and 60 mg/kg) were collected, cut at the middle artery occluded area and then placed into the embedding medium [OCT (optimal cutting temperature)].
In Ruidoso, those are among the uses being proposed by Ms. Barrow, who is working to win approval for a pilot project in which her company would collect cut wood and brush from the ground and would pay a small fee for the rights to the wood.
Furthermore, all data obtained to date on herbaceous species were derived from exudates collected from cuts, incisions or punctures [ 70- 72].
