CM was then collected, concentrated using Agilent Concentrator (Agilent Technologies Inc). and filtered through 0.22 μM Millipore filter.
Extracellular media was collected, concentrated through 100,000 MWCO concentrators (Sartorius), loaded onto 10-60% Iodixanol gradients, and centrifuged at 100,000 g for 16 hours at 4°C.
Fractions containing absorbance peaks were collected, concentrated (∼5×) using Vivaspin 6 mL concentrators (Satorius, Goettingen, Germany) and run on SDS-PAGE and stained with Coomassie Brilliant Blue.
HCV SA13/JFH or mock-electroporated Huh-7.5 supernatants were collected, concentrated through a Vivaspin 100,000 MWCO concentrator, overlaid on 20% sucrose, and centrifuged for 24 hours at 23,000 rpm to purify virus particles.
The conditioned medium was collected, concentrated with a Centricon-10 (UFC910024, Billerica, MA), and protein concentrations were determined with the BCA assay (#23250, Pierce, Rockford, IL).
Conditioned media were collected, concentrated using Amicon Ultra centrifugation filter (MWCO 10K, Millipore), and protein concentrations was determined by Bradford assay (Thermo Scientific; Rockford, IL) according to the manufacturer's instructions.
The enzyme was collected, concentrated and partially purified from the culture supernatant.
Eighty (50 mL) fractions were collected, concentrated and grouped based on TLC profile.
The first peak was collected, concentrated to 5 mg/mL, and screened for crystallization.
Sixty (50 mL) fractions were collected, concentrated and grouped based on TLC profile.
The enzyme fractions were collected, concentrated, and exchanged with 10 mM phosphate buffer (pH 7.0).
