Single experiments in Akita/A1AR-/ (DKO) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in wild type (WT) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Data Summary of measurements of renal function in kidneys and single nephrons of wild type, Akita, and Akita/A1AR-/ mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in Akita mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Following the determination of PI and GI, 4 ml paraffin-stimulated saliva was collected and the collection time was recorded.
For blood samples that were collected at routine collection times, a SCr concentration was already available in the hospital records.
For the environmental nutrition intervention, the menus are collected at each data collection time point and analyzed for nutrients using ESHA software.
Blood was collected at the saliva collection time points by retro-orbital plexus bleeding, from which plasma was separated by centrifugation for five minutes in an eppendorf tube centrifuge.
The multiple weighing technique has advantages of increasing the collection time by collecting large water mass thereby reducing the diverter error, the main source of measurement uncertainty, consequently improving measurement uncertainty of the system.
Information on weight and height prior to pregnancy was collected at the first data collection time point (<25 weeks) via maternal report.
