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The thin As layer collected at the interface can be used in the design of very shallow junctions.
The carbonaceous materials liberated by this treatment were collected at the interface between the CsF-HF solution and the dioxane, rinsed in succession with 2 N HCl, 1 N HCl 9 N HF, 2 N HCl, milliQ water, and methanol, dried at 40°C to 60°C for 12 h, and finally mounted in synthetic resin.
The cells collected at the interface were cultured in alpha-MEM modified with ribonucleosides and deoxyribonucleosides supplemented with 20% fetal calf serum, with 2 mM L-glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin (complete medium) in an atmosphere of 5% CO2 and 95% humidity at 37°C.
Cells were centrifuged at 600 g for 30 min. Mononuclear cells were collected at the interface of the Fico/Lite LymphoH and media, centrifuged at 600 g for 10 min, resuspended in DPBS, and evaluated with a hemacytometer to determine cell concentration and viability (trypan blue exclusion).
Viable cells were collected at the interface and washed with PBS solution.
Lymphocytes were collected at the interface between the two layers by a sterile 1 ml pipette.
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Fractions were collected at the interfaces of the OptiPrep gradient and assayed for the activity of acid phosphatase, a hydrolase highly accumulated in lysosomes, using the acid phosphatase assay kit (Sigma).
Preliminary data indicates that the lipase tended to collect at the interface.
(b) Fourier-filtered enhancement collected at the ZnO/CdTe interface, as depicted in the blue rectangular area in (a).
However, many samples collected at the weathering bedrock interface yield ages in the 51 41 Ma range, suggesting that the weathering profiles in the Quadrilátero Ferrífero had already reached their present depth in the Paleogene.
Myelin membranes were collected at the 0.32 M/0.85Msucrose interface with a Pasteur pipette.
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