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Samples were collected at noon and transported to the laboratory in 20 l polycarbonate bottles (Nalgene, Rochester, NY, USA) within 6 h.
Leaf discs (0.63 cm2 in size) were collected at noon under full sun and immediately frozen in liquid N2.
Embryos were collected at noon of embryonic days.
Leaf discs (0.58 cm2 in size) were collected at noon under full sun and immediately frozen in liquid nitrogen.
AdditionaLeafle 3: Specific primer pairs usediscs qRT-PCR expression analysis.
Samples were collected at noon and midnight in the experiments studying the light/dark variations, and at noon in all other experiments.
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Availability: Very rare, but large last stadia larvae are easily collected at night lights and molt to postlarvae that can be reared.
Blood samples were collected at 7 points: before sternotomy, at 10, 30, and 60 minutes of cardiopulmonary bypass, at termination of cardiopulmonary bypass, 5 minutes after protamine administration, and 2 hours after cardiopulmonary bypass.
Female M. dalli were collected at night from the Swan-Canning Estuary in Perth (31°56′50″S 115°54′58″E), south-western Australia from December 2014 to March 2015, using a hand trawl net that was 1.5 m high, 4 m wide and constructed from 9 mm mesh.
In analyzing spatial and seasonal distribution of zooplankton, we excluded samples collected at night, in order to eliminate the impact of diel vertical migrations, which could cause from a two- to a fivefold increase of zooplankton abundance and biomass (Goswami and Shrivastava 1996; Luo et al. 2000; Smith et al. 1998).
She left the hospital, moved into an abandoned house, lived on apples she collected at night, and eventually died from malnutrition.
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