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Material of approximately 15 fully expanded leaves from at least three different plant individuals per plot and species was collected at midday (12 00 13 30) on 23 May 2008 at estimated maximum canopy development before first mowing.
For SSH and cDNA library construction, shoots were collected at midday (12 00) and midnight (24 00) on Jun 2, 2010 from the same four individuals at each time point (8 samples total).
The normalized cDNA library was constructed from an RNA mixture extracted from shoots collected at midday and midnight to obtain gene sequences expressed extensively in the daytime and nighttime in the summer (Additional file 1).
Samples from all coral fragments were collected at midday after three months (9th July 2010 at Orpheus, 14th July at Keppels) frozen in liquid nitrogen, then transferred into RNAlater (Ambion, Austin, TX, USA) for gene expression profiling.
A normalized cDNA library was constructed using a total RNA mixture consisting of equal amounts of total RNA isolated from samples of the same four individuals collected at midday and midnight.
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Leaf discs (0.63 cm2 in size) were collected at noon under full sun and immediately frozen in liquid N2.
Blood samples were collected at 7 points: before sternotomy, at 10, 30, and 60 minutes of cardiopulmonary bypass, at termination of cardiopulmonary bypass, 5 minutes after protamine administration, and 2 hours after cardiopulmonary bypass.
Female M. dalli were collected at night from the Swan-Canning Estuary in Perth (31°56′50″S 115°54′58″E), south-western Australia from December 2014 to March 2015, using a hand trawl net that was 1.5 m high, 4 m wide and constructed from 9 mm mesh.
In analyzing spatial and seasonal distribution of zooplankton, we excluded samples collected at night, in order to eliminate the impact of diel vertical migrations, which could cause from a two- to a fivefold increase of zooplankton abundance and biomass (Goswami and Shrivastava 1996; Luo et al. 2000; Smith et al. 1998).
She left the hospital, moved into an abandoned house, lived on apples she collected at night, and eventually died from malnutrition.
Acoustic data (38 kHz) were primarily collected at night with one of the clovers and two of the transects surveyed during the day.
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