Coral samples collected as described below were shipped to UNC Wilmington on either dry ice or in liquid nitrogen for RNA extraction, or in 70% ethanol for histology.
Serum and bronchoalveolar lavage fluid (BALF) (collected as described below) were evaluated for antibody levels were by ELISA in plates coated with PspA5.
The QOL data were collected as described below.
During surgery, cancellous bone samples were collected, as described below.
After reperfusion, mice were euthanized, and blood and tissue samples were collected, as described below.
For these patients a broad range of data was collected, as detailed below.
Volatiles collected as described below on Super Q traps (30 mg Super Q, ARS, Gainesville, FL, USA) were extracted by washing with varying amounts of n-hexane (depending on the experiment, see below) containing cuparene as internal standard.
After the pre-feeding period, two fish from each pre-challenge tank, 18 in total (n = 6 per dietary treatment) were collected, as described below, prior to challenge (0 time-point) and the remaining fish were transferred to challenge tanks (1 m).
Maternal height, weight, mid-upper arm circumference, and blood pressure are measured; HIV status, hemoglobin, and urinalysis are tested by point-of-care methods; biospecimens are collected as described below (and in companion article [ 83] and Table 3; equipment used is listed in Table 3 in the Supplementary Appendix).
Inoculated tubers were stored in tightly sealed dark boxes under high (~95%) humidity and at room temperature for 72 hours (during which time all RNA-seq samples were collected as specified below) and then moved to 11 13 Celsius for disease development and phenotyping at 11 days post inoculation.
