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Two reviewers (M.E. and O.T). extracted the following data from all articles collected and resolved disagreements by consensus (21, 26 145; Appendix S1).
The supernatants were collected and resolved on a 7% polyacrylamide gel.
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It was evident that providing abundant material and didactic lectures traced from the introduction to management, and collecting and resolving the questions, promoted comprehension.
BHK-ABCA1 cells were seeded at 0.2×, 1×, and 2× of normal plating density, labeled with [H]choline and [C]cholesterol, treated with a high concentration of mifepristone to induce ABCA1 expression, and exposed to a saturating concentration of human lipid-free apoAI, followed by the regular procedure to collect and resolve lipoprotein particles.
Consequently, such data collection was conducted at a wavelength of 0.9184 Å with 0.4° oscillation angles to maximize the diffraction resolution collected and to resolve the crowded diffraction pattern.
The resulting krypton X-ray fluorescence at 12.65 keV was collected and the spatially resolved signal was subsequently converted into the local temperature of the imaged spot.
Whole-cell lysates were collected and proteins were resolved by SDS-PAGE and were then electrotransferred onto polyvinylidene fluoride membranes.
0.2 ml fractions were collected and aliquots were resolved by SDS-PAGE followed by IB with anti-Rpl32.
RA FLS were collected and proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper (GE Healthcare, Piscataway, NJ, USA).
Cell lysates were centrifuged at 10,000 g for 15 min, the supernatants were collected, and the proteins resolved in 10 % SDS-PAGE gels.
SDS containing β-mercaptoethanol was added to the sample, collected by centrifugation and resolved by SDS-PAGE.
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