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The active fractions were collected, added to ammonium sulfate at 30% saturation, and subjected to a Butyl-TOYOPEARL® 650 M column equilibrated with 30% saturation of ammonium sulfate in same buffer.
On Christmas, Wright -- who told CBS News that this plan had been on his "bucket list" for a while -- took the cash he collected, added hundreds of his own money, then donated $1,000 to the Bethel Mission, a men's emergency shelter in Des Moines.
The unbound sequences were collected, added into the GP73-coated wells, and incubated for 60 min at 37°C.
The supernatant from each group was collected, added into the pre-coated plate of the kit and incubated for 3 hours.
The endothelial cells were stained in medium containing 10 μg ml−1 Di-I (Molecular Probes, Eugene, OR, USA) for 24 h, washed four times with PBS, collected, added into the FluoroBlok inserts (5×10 MHE or HMVEC-L) and incubated for 24 h.
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Cells were collected adding an appropriate volume of mammalian lysis buffer.
Nevertheless, in population-based cancer registries, which prioritise quality over amount of information collected, adding new variables to their dataset has to be carefully considered because each new variable can dramatically increase the workload involved.
From the 4th quarter of 2004 until the end of 2007 a total of 2,943,294 reports were collected adding up to 970,856 when the different versions of the same report are grouped together.
"The drivers would call at 1 in the morning; they'd call collect," added Kenny Lund, vice president of support operations.
We could use the tax system to collect added annual loan principal repayments from anyone whose payments fell below a minimum percentage of their income.
It's these few cents that Apra Amcos collects, adds up, and pays out.
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CEO of Professional Science Editing for Scientists @ prosciediting.com