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The integration time was 10 s, and the number of collected accumulations was from 1 to 10 depending on the sample.
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"This glacier should be in better shape because it collects accumulation during the monsoon.
Spectra were collected with repeated accumulations (2 3), each of 15 150 s (15 s for the yellow, 30 s for the blue, 60 s for the white, and 150 s for the red and green enamels).
The signal from XPS was collected with 5 accumulations for each layer, measuring elemental Al and O.
Spectra were collected by the accumulation of 232 shots at laser intensity 180 in a positive mode.
Each array was read twice and mass focus was set at 4 kDa for first reading and 15 kDa for second reading, sample rate was set at 400 and spectra were collected by the accumulation of 540 shots in the positive mode.
Gorgonian richness was exceptional, but not all species were collected, because the species accumulation curve remained steeply inclined after all surveys.
The samples required for qRT-PCR of different cultivars were obtained from flower buds collected after a chilling accumulation of 400 chilling hours (hours below 7°C) [ 25].
MS/MS spectra were scanned from 100 m/z to 1600 m/z and were collected for a total accumulation time of 200 ms. Former parent ions were excluded for 16 seconds following MS/MS acquisition.
Complete growth and metal accumulation data collected during grain filling are presented as an interactive website (Additional file 2: Mini-website showing Cd, Cu, Fe, Mn, and Zn accumulation in low- and high-Cd near-isogenic lines of durum wheat during grain filling).
At the end of the experiment, cells were fixed and stained with Oil Red O to visualize lipid accumulation, or collected for RNA extraction, followed by QPCR [quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR)] for gene expression analysis as described previously (Li et al. 2011).
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