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Blood was taken from the antecubital vein and drawn into 10 mL evacuated tubes with heparin used for plasma collection and non-treated tubes were used to collect serum Samples were centrifuged at 1200 rpm for 10 min and the serum was aliquoted into 1.5 mL polyethylene microcentrifuge tubes and stored at −80°C for subsequent analysis.
Between 30 min and 2 h after collection, blood was centrifuged at 3000 g for 10 min to collect serum and plasma and immediately processed for analysis or aliquoted and frozen at −80 °C until use.
Initially, all households were visited to collect information on dog ecology and demography based on WHO guidelines and to collect serum for rabies antibody detection.
Following an anesthetic over dose with euthasol, rodents underwent a terminal retro-orbital eye bleed to collect serum for subsequent ELISA of hASM.
The remaining fresh blood was placed into a sterile propylene tube, left overnight at 5°C, then centrifuged (2,000 x g at 5°C, 5 min) to collect serum which was stored at −80°C.
Animals in these latter groups were sacrificed 3 10 days later to collect TLN, blood, serum, and nasal turbinate samples or were used to collect serum samples for up to three weeks after the second vaccination.
Similar(32)
Stable transfected HEK-293 cells were used to collect serum-free conditioned media medium (∼1 liter) was concentrated (×10) with a 10.000 MWCO Vivaflow 50 filter (Vivascience AG, Hannover, Germany), dialyzed against PBS (pH 7.4) and loaded onto a HisTrap HP 1 ml column using an ÄKTA Prime plus system (GE Healthcare, Uppsala, Sweden).
The collected serum was stored at –20°C for further assays.
It took three weeks, and most of that time was spent collecting serum from an elusive herd of alpacas.
Blood samples were collected by retro orbital puncture and centrifuged at 1000×g for 15 min and the collected serum was stored at −80 °C until analysis.
We collected serum samples also every 6 h (urea, potassium and sodium levels, troponin, hemoglobin, platelets, C-reactive protein and lactates).
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