K562 cell pellets were collected, fixed with 1.6% glutaraldehyde, post-fixed in 1% OsO4, dehydrated in alcohol series, and embedded in epoxy resin.
Several time points interested were chose and the corresponding cell samples were collected, fixed with formaldehyde and stained with DAPI.
Lungs were collected, fixed and embedded into paraffin.
Cells were collected, fixed, and permeabilized according to manufacturer's instructions (Caltag Laboratories, Burlingame, CA, USA).
They were collected, fixed, and incubated with a FITC-conjugated monoclonal antibody against BrdU.
Cells were collected, fixed, stained and FACS analysis was performed as described previously [20].
Brains of euthanized mice were collected, fixed in 4% paraformaldehyde, paraffin embedded, sectioned, and subjected to hematoxylin and eosin staining.
After one hour, all embryos from the litter were collected, fixed, embedded in paraffin, and sectioned with a microtome.
As controls we also collected fixed, stained hESCs using the same SOX17 GATA4 CXCR4 three-channel protocol, but without sorting.
At necropsy, colon was collected, fixed in 10% formalin, paraffin embedded, sectioned at 5 µm, and stained with hemoxylin and eosin for histologic evaluation.
8 mm diameter skin biopsy samples centred on the wound bed were collected, fixed in 10% formalin for a maximum of 48 hours and embedded in paraffin.
