Sentence examples for collect conditioned from inspiring English sources

To collect conditioned media (CM), the media was changed to serum-free medium after 6 hours of co-culture and CM was collected 12 hours after media change.

To collect conditioned media, cells were grown to 100% confluence, and a week later regular media was substituted for a low serum (3%) media for 72 hours.

To collect conditioned medium, indicated cells were cultured to confluency, washed twice in DMEM and cultured for a further 24 hours in defined medium (DMEM containing 100 µg/ml transferrin, 100 µg/ml BSA, 60 ng/ml progesterone, 50 ng/ml thyroxine, 50 ng/ml tri-iodothyrine, 16 µg/ml putrescine and 40 ng/ml selenium).

To collect conditioned medium (CM), cells were grown to 90% confluency in 100 mm culture dishes in DMEM plus serum.

After co-culture with MECs, SC were re-plated and grown alone to collect conditioned medium, and secreted MMP-9 or MMP-2 activity was examined by zymography (Tokar et al. 2005).

In order to collect conditioned medium on which we tested metalloproteinase and urokinase activities, cell cultures were allowed to grow in complete medium for 6 days in presence of SE, CR, and CCT.

The collagen cleavage activity of collected conditioned media was dramatically inhibited by STI but not by 1,10-phenanthroline, an MMP inhibitor.

To assess if promoted migration was due to TGFβ secretion, we collected conditioned medium from wild type and Ikkβ −/− cultures and examined its effects on migration of the wild type cells.