Questions were asked via drop and collect self-completion questionnaires.
Those who agreed to take part in the study were given the questionnaires, which were completed anonymously and collected after completion.
Data were collected by completion of a standardized case record form.
The papers are collected upon completion of the test and cannot be taken home by the examinees.
Samples collected after completion of flow-through experiments were used to determine methane oxidation kinetic parameters, Vmax, Km, and methanotrophic community distribution across biofilter columns.
Animals were sampled seven times over 2 years and were classified as positive if they had persistent agglutination titers or had Brucella isolated from specimens collected at completion of the study.
The liquid products were collected after completion of the run and analyzed by means of three gas chromatographs, a Shimadzu 4C gas chromatograph (Shimadzu Corp., Kyoto, Japan) equipped with two subsequently connected packed columns: Porapak Q (Sigma-Aldrich Corp., St . Louis MO, USA) and molecular sieve 5 Å, and a TCD with argon which was used as a carrier gas for hydrogen analysis.
The products were collected after completion of the run and analyzed by means of three gas chromatographs, a Shimadzu 4C gas chromatograph (Nakagyo-ku, Kyoto, Japan) equipped with two subsequent connected packed columns: Porapak Q and Molecular Sieve 5 Å, and a TCD with argon which was used as a carrier gas for hydrogen analysis.
The liquid products were collected after completion of the run and analyzed by means of three gas chromatographs, a Shimadzu 4C gas chromatograph (Shimadzu Corp., Kyoto, Japan) equipped with two subsequent connected packed columns: Porapak Q (Sigma-Aldrich Corp., St . Louis MO, USA) and molecular sieve 5Å, and a TCD with argon which was used as a carrier gas for hydrogen analysis.
In the case of A. australis, several blood samples (n = 15) were collected after completion of the PHA assay (see results).
