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collect HPIs for the previous interval.
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The root tissues were collected 4 hpi and MtN5 expression was checked by quantitative RT-PCR (qRT-PCR) analysis.
At 24 HPI and 48 HPI, the cells were collected for RNA extraction with the TRIzol reagent (Invitrogen Life Technologies, USA).
At present, VirHostNet has only seven HPIs for BVDV and three HPIs for BHV, with very limited number of HPIs.
At 72 hpi lesions were collected and used for RNA extraction.
Leaves were collected at 3,6,9 and 18 hpi for RNA gel blot analysis.
The cell supernatants were collected and tested for IFN-β production 24 hpi.
The culture was synchronized with 5%% sorbitol and the parasites were collected at 18, 30 and 40 hpi for chromatin immunoprecipitation.
At 0, 3, 6, 12, 24, 36, 48 and 72 h post-injection (hpi), five shrimp from each group were randomly selected, and the hemocyte, gill, hepatopancreas and intestine were collected for quantitative PCR (qPCR) analysis.
The infected silkworm's haemolymph was collected every 12 h starting at 60 hpi for determining the expression course of target antigen.
For RNA-Seq analysis, seeds were collected at 72 hpi.
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