Sentence examples for colder solution from inspiring English sources

A citrulline calibration curve was performed by mixing 100 µL of 0, 3.125, 6.25, 12.5, 25, 50, 100, 200 or 400 µM citrulline in 100 mM Tris buffer containing 2 mM reduced glutathione with 300 µL COLDER solution, and subsequently treated as described above.

For color development, 200 μL of freshly prepared COLDER solution (2.25 M H3PO4, 4.5 M H2SO4, 1.5 mM NH4Fe SO4), 20 mM diacetyl monoxime, and 1.5 mM thiosemicarbazide) was added to the quenched reaction, and the mixture was vortexed to ensure complete mixing and then incubated for 30 min at 95 °C.

There is also a cold solution.

To the cold solution it was added EDCI.HCl (5.5 mmol, 1.1 equiv ., HOBt (5.5 mmol, 1.1 equiv).

The deactivated surfaces of the NPs together with the cold solution significantly slow the random growth of the NPs by OR and OA processes.

The Fmoc-Lys(ivDde -OH was divDde -OHin 2 mL of DMF at 0°C, and HATU (2.5 equivalents) was adissolvedhe cold solutinn.

The Fmoc-NH- PEG 1-CO2H (2.5 equivalents) was dissolved in 2 mL oFmoc-NH- PEG 1-CO2H(2.5 Fmoc-NH- PEG 1-CO2HBOP (2.5 equivalents) wase adissolvedhe cold solutinn.

The endovascular cooling catheter contains a circulating cold solution, which is maintained at a controlled temperature; this method can easily achieve a cooling rate of 1.5 4.5 °C/h [52].

The reaction was arrested with excess ice-cold solution and cells were washed.

The tissues were fixed in a freshly prepared ice-cold solution containing 2.5% glutaraldehyde and 4% formaldehyde in 0.1 M PBS pH 7 for three hours.

Slices were prepared in ice-cold solution using a HM 650 V vibroslicer (Microm, Germany), incubated at 35°C for 40 minutes and maintained at room temperature.