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Then the cells were washed once in ice-cold 1 × PBS and resuspended at 1 × 105 cells mL−1 in ice-cold 1 × PBS.
The third coldest, 34.6 degrees, was in 1985.
That's pretty cold; -459, after all, is absolute zero.
When the mixture is cold (4C), mix in the espresso.
There, CBS won every-half hour, with repeats of "60 Minutes" (10.77 million), "Cold Case" (10.44 million) and a made-for-television movie, "Stone Cold" (9.79 million).
Colorado (US) will stay cold (–9C by day, –20C at night).
It was during this period that he fashioned the G-funk sound, said Cold 187um.
Clouds of nasty gases reflect away sunlight, leaving the surface at a blisteringly-cold -179C.
It's dark (winter runs through November), cold (65 below) and windy.
She doesn't mind height, but it can be very cold 40 stories off the ground.
In fact, Cold 187um says he wasn't slated to produce any new NWA tracks.
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