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Immediately after slaughter, samples of longissimus lumborum muscle and SAT for gene expression analysis were collected from the right side of carcass at the 5th lumbar vertebra level, rinsed with sterile RNAse-free cold water solution, cut into small pieces (thickness of ~0.3 cm), stabilised in RNA Later solution (Qiagen, Hilden, Germany) and subsequently stored at −80°C.
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Figure 7b shows the ABE concentrations in the cold-water solution.
In the experiment, both condensate and cold-water solution were collected and measured.
No ethanol was detected in the cold-water solution indicating that all evaporated ethanol was condensed.
The acetone concentration in the cold-water solution was exceptionally high compared to the butanol.
The condensation system was unable to effectively trap the acetone; therefore, a substantial fraction of acetone (44%%) was captured in the cold-water solution.
Vacuum pump exhaust (flue gas) was bubbled in a 300-mL cold-water solution chilled with iced water, to collect escaping (uncondensed) ABE vapors.
For butanol, 80%% were recovered in the condensate, 17%% remained in the fermentation broth, and only 4%% captured in the cold-water solution.
By combining the ABE products in the fermentation broth, the condensate, and the cold-water solution, a total of 15.1 g ABE was produced, of which acetone, ethanol and butanol were 3.1, 0.9 and 11.1 g, respectively.
In this integrated vacuum fermentation system, 17 % of the produced ABE remained in the fermentation broth, 71%and12%12 % were recovered in the condensate and the cold-water solution, respectively.
If the cold-water solutions don't help enough, over-the-counter products may.
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CEO of Professional Science Editing for Scientists @ prosciediting.com