There's a possibility, for example, that cold tissue needs less oxygen to begin with.
Regression slopes of grain density versus concentration for calibration standards were the same for sections placed on cold tissue or glass.
The cold tissue was then cut into rings of roughly 1 mm in thickness using a pair of sterilized safety razor blades.
If your eyes are itchy and swollen, place a damp, cold tissue or wash cloth over each eye.
Ice-cold tissues were cut into small pieces (Ø <4 mm) and spiked with pipetted droplets of culture of known concentration.
The cell pellet was resuspended in buffer I (20 mM Tris-HCl, pH 7.6), 15 mM magnesium acetate, 100 mM ammonium acetate, and 6 mM 2-mercaptoethanol containing 1 mM phenylmethylsulphonyl fluoride (PMSF), and lysed by vortexing with 0.2 mg glass beads in an ice-cold Tissue lyser (Qiagen, Venlo, The Netherlands).
The medium overlying the THP-1 monolayer was replaced with 1 ml of ice-cold tissue culture medium.
Cells were homogenised in an ice-cold tissue grinder with 10 strokes.
A surgical laparotomy was performed, and the uterine horn was removed and placed into ice-cold tissue collection buffer.
Regions were weighed and homogenized in 1% (w/v) ice-cold tissue protein extraction reagent (T-PER) (Thermo Scientific, Rockford, IL) with 1% Halt protease inhibitor cocktail (Thermo Scientific) and 1% phosphatase inhibitor cocktail (Sigma-Aldrich, St . Louis MO, USA).
The biopsies were then placed in an ice-cold tissue collection buffer (TCB) (119 mM NaCl, 4.7 mM KCl, 1.6nM CaCl2H20H24, 24 NaHCO3, 1.18 KH2PO4, 1.2 MgSO4, 0.05 EDTA, 5.5 glucose at pH 7.4) and remained on ice until studied.
