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After prolonged cold storage, fruits produce a woolly phenotype when ripened.
Increased transcripts that correspond to this contig were detected when comparing long-term cold storage fruits (E3: R-, C-) with fruits from packing (E1: R-, C-).
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This work demonstrates that during cold storage, peach fruits differentially express genes that are associated with cold acclimation.
However, prolonged cold storage of fruits such as peaches and nectarines can trigger a physiological disorder known as chilling injury or internal breakdown, which negatively affects fruit quality [ 1- 3].
Hypobaric storage involves the cold storage of fruit under partial vacuum.
Furthermore, correlating with ethylene production, the gene and protein expression of the ACO and ACS1were depleted during cold storage in fruit developing WLT during shelf life [ 27, 28].
We sequenced and statistically analyzed the abundance of Expressed Sequence Tags (ESTs) from peaches at four different post-harvest conditions, mimicking the stages of cold storage for fruit exportation.
These genes were constitutively expressed at high levels in the tolerant group of fruit and down regulated during cold storage in sensitive fruit, while in tolerant fruit they were less affected or even not changed (k-means 5 and 10; Figure 6).
The remaining 94 contigs that are differentially expressed between unripe fruits stored at 4°C (E3: R-, C+) and unripe fruits prior to storage (E1: R-, C-) are novel and may participate in the adaptation and/or stress responses associated with long-term cold storage of these fruits.
A lack of ethylene production during cold storage affects normal fruit ripening and leads to WLT [ 28].
EIN2 (ETHYLENE INSENSITIVE2) has been previously reported during cold storage in peach fruit [ 66] and it has been associated with cold sensitivity in Arabidopsis [ 67] and peach [ 17].
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