Sentence examples for cold stop solution from inspiring English sources

Transport was stopped by washing with an excess volume of cold stop solution.

150 μL ice cold stop solution was added and the absorbance read at 490 nm.

150 μL ice cold stop solution (4N H2SO4) was added to 2 of the sample wells.

150 μL ice cold stop solution (4N H2SO4 + 2 mM resorcinol) was added to 2 of the sample wells.

Immediately after harvesting culture extracts, a one-fifth volume of an ice-cold stop solution (5% phenol in ethanol) was added to prevent RNA degradation [ 48].

The ice-cold stop solution rapidly cools the reaction sample and raises the pH to a level where amylase is no longer active.

The stopped reaction mixture was filtered on a 0.45 μm Millipore (HAWP) filter and washed with 10 mL ice-cold stop solution, twice.

At time intervals (30 s, 60 s, 90 s, etc), adding 5 ml of ice-cold stop solution containing 150 mmol/l KSCN and 10 mmol/l Tris-Hepes (pH 7.4) ended the incubation.

Reactions were carried out at 37°C for 10 min and were stopped by the addition of 3 mL of ice-cold stop solution (0.25 mol/L sucrose, 100 mmol/L NaCl, and 10 mmol/L Tris-HCl, pH 7.4).

The filters were washed 3 times with 3 mL of ice-cold stop solution and dried at room temperature for 30 min. Radioactivity was measured with a liquid scintillation counter.

Immediately, we added 4 ml of ice-cold stop solution (25 mM sodium citrate buffer, pH 4.5) transferred onto glass fiber filters and washed with an additional 4 ml of stop solution.